The mechanism of influence of kaempferol on proline-rich acidic protein 1 (PRAP1) expression after percutaneous coronary intervention (PCI) was investigated based on sini decoction and siwu decoction

Abstract

Coronary artery disease (CAD) is a common cardiovascular disease that is characterized by accumulation of fatty deposits. Recombinant Human Proline-Rich Acidic Protein 1 (PRAP1) expression is related to prognosis of patients after percutaneous coronary intervention (PCI). This study will be aimed at the treatment by the traditional chinese medicine Sini Decoction (SND) and Siwu Decoction (SWD), and investigate the influence of kaempferol in dried ginger on the postoperative coronary PCI, and further explore the mechanism of kaempferol on the expression of Proline-rich acidic protein 1 (PRAP1) after coronary PCI. Mesenchymal stem cells (MSCs) were isolated and induced to differentiate into endothelial progenitor cells (EPCs). After identification of EPCs by immunofluorescence and angiogenesis assay, cells were divided into high concentration of SND combined with SWD group, low concentration group, vehicle group, and negative control group. Immunofluorescence and Western blot were used to determine the expressions of β-catenin and GSK-3β as well as PRAP1 in EPCs, whilst cell migration, proliferation and adhesion abilities were assessed. MSCs were positive for CD105 and negative for CD34 and CD45, followed by identification of EPCs with staining. Regardless of concentration, administration of SND plus SWD significantly increased EPC migration and proliferation, but decreased adhesion rate of EPCs (P <0.05). Moreover, high concentration of SND and SWD significantly facilitated EPC growth and reduced cell adhesion (P <0.05). Importantly, the levels of PRAP1 and GSK-3β were elevated, and β-catenin decreased in the presence of SND and SWD, with high concentration achieving more significant alterations than low concentration. EPCs were fluorescently stained and showed proliferative properties and in vitro angiogenesis. Sini Decoction and Siwu Decoction can significantly increase β-catenin expression and decrease GSK-3β and PRAP1 expression after PCI. Sini Decoction and Siwu Decoction can also promote cell migration and cell proliferation, and significantly reduce the adhesion ability of EPCs, so as to increase new blood vessels, improve cardiac function and protect the heart.