Affiliation:
1. Hunan Key Laboratory of Biomedical Nanomaterials and Devices, Hunan University of Technology, Zhuzhou, 412007, China
Abstract
Cadmium ion (Cd2+) is one of common environmental pollutants, which can be enriched in organisms through the food chain and eventually cause chronic toxicity in humans. Therefore, a strict monitoring tool for Cd2+ is highly required. In this study, we screened
the specific candidate aptamers of Cd2+ based on magnetic beadssystematic evolution of ligands by exponential enrichment method (Mag-SELEX). Firstly, a high enrichment ssDNA library was obtained after eight selection rounds. Then the affinity of fifteen candidate aptamers was compared,
and found that Cd-14 had highest affinity with dissociation constant (Kd) of 23.02±3.53 μM. The Cd-14 was applied to an electrochemical aptasensor based on black phosphorus/Poly-L-Lysine with a linear range of 0.001–100 nM and good specificity.
Publisher
American Scientific Publishers
Subject
General Materials Science
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