Effect of Matrigel on the Culture of Human Induced Pluripotent Stem Cells on Polycaprolactone to Prepare Cell Patches

Abstract

The low seeding rate and survival rate of transplanted cells is one of the difficulties of stem cell transplantation in the treatment of myocardial infarction. By culturing human induced pluripotent stem cells (hiPSCs) on Matrigelcoated polycaprolactone (PCL) in vitro to mimic the natural extracellular matrix in terms of composition and structure, we investigated the effect of Matrigel on the adhesion and proliferation of hiPSCs on PCL. After resuscitation, culture, and identification of hiPSCs, the cells were seeded on Matrigel-coated plastic, PCL, or Matrigel-coated PCL. After 24 h, cell growth was observed by 4,6-diamidino-2-phenylindole (DAPI) fluorescence under a fluorescence microscope and hiPSC stemness was identified by OCT4 fluorescence. After fixation, scanning electron microscopy was performed to observe the surface morphology of cell patches. A growth curve was used to assess cell proliferation. The results showed that hiPSCs can be cultured on PCL and Matrigel-coated PCL to prepare cell patches. Matrigel-coated PCL provides a better environment for cell growth, adhesion, and proliferation. Thus, Matrigel is effective for the culture of hiPSCs on polycaprolactone to prepare cell patches.