Lipid Nanoemulsion Regulates Parkinson’s Cell Cycle and Proliferation Through miR-199/NF-κB Pathway

Abstract

Abnormal expression of miR-199 is related to proliferation of Parkinson’s cells. Lipid nano emulsions can effectively inhibit cell growth. This study mainly explored whether lipid nano emulsions may regulate the expression of miR-199, thereby affecting Parkinson’s cells. SH-SY5Y Parkinson’s cells treated with 20 μmol/L miR-199 in SH-SY5Y cells, or both 20 μmol/L lipid nanoemulsion were used to intervene SH-SY5Y cells. Intervention time was set at 0, 6, 12, and 24 h, respectively. We used 20 μmol/L lipid nano emulsion to interfere with SH-SY5Y cells that were transfected with miR-199. We observed proliferation number and cell cycle changes after lipid nanoemulsion intervention in the SH-SY5Y cells. We also observed the expression of NF-κB pathway factor protein and verified that P65 is the direct downstream of miR-199. The higher the concentration of lipid nanoemulsion was, the more significant the decreased cell proliferation. The concentration of lipid nanoemulsion was 20 μmol/L, which decreased obviously (P < 0.01). Cell numbers in 20 μmol/L curcumin lipid nanoemulsion began to show an evident downward trend from 24 h, where cells from the G0/G1 phase increased and those in G2/M phase decreased significantly (P < 0.05). The higher the dose of lipid nanoemulsion, the higher the miR-199 expression, which showed a concentration and dose-dependence. The number of cells in the lipid nanoemulsion+miR-NC group was lowest. NF-κB is the direct target gene of miR-199. When the lipid nanoemulsion concentration was 20 μmol/L, the expression of P65 protein decreased. Lipid nano emulsion can therefore inhibit proliferation of Parkinson’s cells, and cell number in the S phase and G2/M phase, so that many cells are blocked in the G0/G1 phase, which then control cell growth. Lipid nano emulsion works mainly through miR-199/NF-κB axis, where miR-199 negatively regulates the NF-κB pathway, and ultimately controls abnormal cell proliferation.