Research on Mechanism of Hypoxia-Inducible Factor-1α Gene Carried with Albumin Nanoparticles in Regulating Tumor Angiogenesis in Human Papillomavirus Through Induction of PI3K/AKT Signal Pathway

Abstract

The aim of this study was assessing the mechanism of HIF-1α gene carried with albumin nanoparticles in regulating the tumor angiogenesis in HPV through induction of PI3K/AKT signal pathway. 40 of Wistar rats were divided into blank set, HIF-1α set, agonist set and inhibitor set randomly. There were 10 rats in every set. The gene expression of HIF-1α, presentation rate of CD31 and SMA, protein expression of VEGF and TSP-1, and presentation of factors related with PI3K/AKT signal pathway were all studied. HIF-1α gene carried with albumin nanoparticles was prepared successfully, and the gene expression of HIF-1α in HIF-1α set was highest. The second highest expression was in the inhibitor set, while the lowest was in the blank and agonist sets. There were notably-visible tan particles in the tumor tissue from the blank, HIF-1α and agonist sets, and they were reduced notably in the inhibitor set. The presentation rates for CD31 and SMA in the tumor tissues from the blank, HIF-1α and agonist sets were highest, while the rate was reduced in the inhibitor set. The action on VEGF protein expression was similar, and there was positive correlation between abnormal activation of HIF-1α and tumor angiogenesis in HPV. The PI3k/AKT signal transduction pathway was activated by HIF-1α and PI3k and AKI were phosphorylated. The HIF-1α was prompted to enter into cell nucleus and interact with element with low oxygen effect so as to increase the presentation of VEGF, CD31 and SMA. The angiogenesis in the HPV was also prompted, but the action was eliminated by inhibitor from the PI3K/AKT pathway.