Highly Fluorescent N-Doped Carbon Quantum Dots Derived from Bamboo Stems for Selective Detection of Fe3+ Ions in Biological Systems

Abstract

The establishment of sensing platform for trace analysis of Fe3+ in biological systems is meaningful for health monitoring. Herein, a Fe3+ sensitive fluorescent nanoprobe was constructed based on highly fluorescent N-doped carbon quantum dots (NCQDs) derived from bamboo stems through a hydrothermal method employing ethylenediamine as the nitrogen dopant. The prepared NCQDs had a uniformly distributed size and their mean size was around 2.43 nm. Abundant functional groups (C=N, N-H, C=O, and carboxyl) anchored on NCQDs demonstrated successful doping of N in CQDs. The obtained NCQDs possessed a high fluorescence quantum yield of 20.02% and outstanding fluorescence stability over a wide pH range and at high ionic strengths. Moreover, Fe3+ ions presented a specific fluorescent quenching effect to the as-prepared NCQDs. The calibration curve for fluorescence quenching degree corresponding to Fe3+ concentration showed a linear response in a range of 0.01–10 µM, and detection limit was 0.486 µM, which indicated that the NCQDs had high sensitivity to Fe3+ ions. Ascribed to these unique properties, the NCQDs were selected as luminescent probes for trace amount of Fe3+ ions in human serum. These results demonstrated their promising use in clinical diagnostics and other biologically relevant studies.