Preparation of Polymer Nanocarrier Material and Its Application in B-Cell Lymphoma

Abstract

The β-cyclodextrin (β-CD) is coupled with polyethyleneimine (PEI 600 Da) to produce a polymer nanocarrier material (PyD-W) with good biocompatibility and high transfection rate. First, the test was performed to study the influence of different factors on the transfection efficiency of PyD-W materials in terms of cell type and transfection system. Then the effect of adding wheat germ agglutinin on the material-cell membrane binding when transfecting cells by PyD-W materials was studied. The influence of temperature and cell phagocytosis inhibitors on the entire transfection process were taken into consideration at the same time. In the end, the escape ability of PyD-W material carrying plasmid DNA in lysosomes was studied. After analyzing the performance of PyD-W material transfected cells, the appropriate transfection staining conditions was selected to be applied in the study of the inhibitory properties of mouse B-cell lymphoma cells. In the experiment, the results showed that the cell type had a great influence on the material. The N/P ratio should not be set too high. Prolong the transfecting and fostering time appropriately could increase the transfection efficiency. The addition of wheat germ lectin would significantly reduce the enrichment ability on the cell surface of plasmid DNA carried by PyD-W. Increasing the temperature could also increase the transfection efficiency of PyD-W materials, cell phagocytosis inhibitors would not have a significant impact on transfection, and the accumulation of PyD-W materials in lysosomes would be gradually released from lysosomes with time going by. According to the above results, PyD-W carrying plasmid DNA was transfected into mouse B lymphoma cells and normal B cells using similar transfection methods. The results show that B lymphoma cells (38B9) corresponded significant mRNA levels is lower than the mRNA level of normal B cells (P < 0.05). It is detected by cell count and CCK-8 kit that the growth of cells in the group overexpressing PyD-W is significantly inhibited (P < 0.01).