Assessment of the BRAF status in patients with skin melanoma

Author:

Gyrylova S N,Ruksha T G,Zobova S N,Komina A V,Aksenenko M B,GYRYLOVA S N,RUKSHA T G,ZOBOVA S N,KOMINA A V,AKSENENKO M B

Abstract

Skin melanoma is an aggressive malignant neoplasm. Mutation of the BRAF gene, BRAF V600E, is observed in more than 70% of melanoma cases. Assessment of the BRAF status in patients suffering from skin melanoma is an important stage in patient screening for selection of further therapy. To reveal the mutation of BRAF V600E, 27 histology samples taken from patients with skin melanoma residing in the Krasnodar territory were examined by means of defining the Restriction Fragment Length Polymorphism (PFLP assay). Our studies revealed the BRAF V600E mutation in 26 of 27 histology samples of skin melanoma. It was shown that the mutation is not associated with any sex-related, age-related, clinical and pathological features of the tumor.

Publisher

Rossijskoe Obschestvo Dermatovenerologov i Kosmetologov

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