DNA Isolation From Six Spotted Ladybird Beetle <i>Cheilomenes Sexmaculata</i> (F)

Abstract

DNA extraction is a routine step in many insect molecular studies and extraction methods need to be evaluated for their efficiency, cost, and side effects, such as DNA degradation. Individuals of six spotted ladybird beetle, Cheilomenes sexmaculata (F.) were subjected to DNA extraction by the CTAB method and PureLink® Kit. The methods were compared in terms of DNA quantity and quality, cost of materials, and time consumed. The CTAB method resulted in higher DNA yield (ng DNA) at a much lower price and less degradation, as revealed on agarose gels. The PureLink® Kit was the most time-efficient but costliest, and degradation was observed on agarose gels. The DNA samples obtained were tested on agarose gel PCR for six SSRs located in various positions of the beetle’s genome. The results revealed that DNA isolated from the CTAB method showed successful amplifications, but the PureLink® Kit method did not show any amplification. These evaluations guide the choice of DNA extraction methods from C. sexmaculata beetles.