Author:
Paluzar Hatice, ,Sagiroglu Ayten,
Abstract
The present study was aimed to assess the inhibition effects of organophosphate pesticides, malathionR, dichlorvosR; pyrethroid pesticides, deltamethrinR, λ-cyhalothrinR on antioxidantenzymes and reactivation ability of pralidoxime against pesticide inhibited-antioxidant enzymes. Oximes were reported by reactivation ability against organophosphate inhibited- acetylcholinesterase and we focused to investigate the reactivation effect of pralidoxime against organophosphate inhibited–antioxidant enzymes. IC50 values were determined by means of activity percentage diagrams. The concentrations of deltamethrinR, malathionR,dichlorvosR, λ-cyhalothrinR that inhibited 50% of catalase were 5.2 μM, 158 μM, 133 μM,320 μM, respectively, inhibited 50% of superoxide dismutase were 62 μM, 240 μM, 328 μM, 2320 μM, respectively and inhibited 50% of glutathione peroxidase were 0.7 μM, 1198 μM, 1638 μM, 98 μM, respectively. All pesticide doses showed inhibition effect on antioxidant enzymes. DeltamethrinR was found to be a more potent inhibitor for the antioxidant enzymesfollowed by the rest of pesticides used in this study. Reactivation effect of pralidoxime was determined for organophosphate inhibited-enzymes. Reactivation results showed that only catalase is reactivated by pralidoxime against dichlorvosR and malathionR. Under the exposureof 50-800 μM malathionR concentrations, catalase activity % was calculated as 72-11%,respectively. After inhibited catalase by malathionR incubated with 1 mM and 10 mMpralidoxime, catalase activity % was calculated as 92-31% and 98-39%, respectively. Under the exposure of 100-1500 μM dichlorvosR concentrations, catalase activity % was calculatedas 50-6%, respectively. After inhibited catalase by dichlorvosR incubated with 1 mM and 10mM pralidoxime, catalase activity % was calculated as 95-30% and 93-28%, respectively. When the results are examined, it is seen that increasing the pralidoxime concentration does not significantly affect the reactivation percentage of the catalase enzyme.
Publisher
Kuwait Journal of Science
Cited by
2 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献