Phenological Fluctuations of Secondary Metabolites in Dracocephalum charkeviczii

Author:

Grigorchuk V. P.1,Nakonechnaya O. V.1,Grishchenko O. V.1,Bezdelev A. B.2

Affiliation:

1. Federal Scientific Center of the East Asia Terrestrial Biodiversity, Far Eastern Branch, Russian Academy of Sciences

2. Zhirmunsky National Scientific Center of Marine Biology, Primorsky Aquarium Scientific and Educational Centre, Far Eastern Branch, Russian Academy of Sciences

Abstract

Plants from the genus Dracocephalum are a source of biologically active compounds, including rosmarinic acid and different flavonoids. Their concentration varies during the vegetation period. In order to examine changes in their content in Dracocephalum charkeviczii Prob., an endemic species of Sikhote Alin and South Kuriles, wild and cultivated plants were collected in three phenological stages: vegetation, flowering/start of fructification, and preparation for withering. By means of HPLC with UV and mass selective detection, 15 polyphenol compounds were detected in methanol extracts from the leaves. Several new compounds—coumaric acid glycoside, quercetin glycoside and rutinoside, and acacetin coumaroylglycoside—were identified in D. charkeviczii. Synthesis of most flavonoids was found to be the highest in the beginning of the vegetation period, and gradually decreased by its end. The concentration of caffeic acid derivatives (chlorogenic acid, rosmarinic acid glycoside, and dehydrorhabdosiin) increased, but the total concentration of compounds decreased by the end of vegetation.

Publisher

The Russian Academy of Sciences

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