Early Blight Resistance of Transgenic Potato Plants Expressingthe ProSmAMP1 Gene for Antimicrobial Peptides under the Control of a Light-Inducible Cab Promoter

Author:

Beliaev D. V.1,Yourieva N. O.1,Tereshonok D. V.1,Derevyagina M. K.2,Meleshin A. A.2

Affiliation:

1. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences

2. Russian Potato Research Centre

Abstract

The genome of Stellaria media contains a gene family for hevein-like antimicrobial peptides, some of which are known to encode two peptides released from the translation product as a result of post-translational proteolysis. These peptides have been shown to inhibit the growth of bacteria and fungi, including potato pathogens Alternaria solani and Alternaria alternata. One of these genes, ProSmAMP1, was introduced into the potato genome under the control of the light-inducible promoter of Cab gene from common wheat. The resulting transgenic lines expressed ProSmAMP1 mRNA during several vegetative passages, and their resistance to early blight was assessed by several indicators of detached leaf infection, with plants having the highest expression of the transgene also showing the highest resistance.

Publisher

The Russian Academy of Sciences

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