The Effect of Synthetic Polycation Poly-2-Dimethylaminoethylmethacrylate on Biological Activity of Mammalian Resident and Nonresident Cells-Reference-Cited by-同舟云学术

The Effect of Synthetic Polycation Poly-2-Dimethylaminoethylmethacrylate on Biological Activity of Mammalian Resident and Nonresident Cells

Published:2023-09-01 Issue:5 Volume:65 Page:447-460
ISSN:0041-3771
Container-title:Цитология
language:
Short-container-title:Citologiâ

Author:

Ivanova V. P.¹,Alekseenko L. L.²,Nazarova O. V.³,Mindukshev I. V.¹

Affiliation:

1. Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences

2. Insitute of Cytology, Russian Academy of Sciences

3. Institute of Macromolecular Compounds, Russian Academy of Sciences

Abstract

Cationic polymers are the positively charged macromolecules that have in their structure N-containing functional groups such as primary, secondary and tertiary amine groups; quaternary ammonium groups and others. The effect of synthetic polycation poly-2-dimethylaminoethylmethacrylate (PDMAEM) on biological activity of animal fibroblasts (CHL V-79 RJK) and human red blood cells (RBCs) was studied. The influence of PDMAEM on cell adhesion using fibroblast culture was analyzed. Cultural plastic treated or untreated by polycation was used as substrate. The polycation adsorption on polystyrene surface did not change the adhesive capacity of fibroblasts. Pretreatment of fibroblasts with PDMAEM did not influence at low concentrations (0.1 and 1 μg/mL) the adhesive properties of cells plated on the untreated plastic surface. At high concentrations (10 and 100 μg/mL) PDMAEM inhibited the attachment of fibroblasts to this substrate. Relationship between the inhibition of cell adhesion under PDMAEM action and the toxic effect on fibroblast viability has been found. The PDMAEM treatment of human RBCs at high doses led to the damage of cells and release of hemoglobin to incubation medium. At low doses PDMAEM practically did not influence the hemolysis of RBCs. It was shown that PDMAEM induced the change of the shape and aggregation of RBCs. The toxic effect of PDMAEM on human RBCs coincided as a whole with such effect for animal fibroblasts. Possible cell targets upon the PDMAEM effect are discussed.

Publisher

The Russian Academy of Sciences

Reference56 articles.

1. Боровская М.К., Кузнецова Э.Э., Горохова В.Г., Корякина Л.Б., Курильская Т.Е., Пивоваров Ю.И. 2010. Структурно-функциональная характеристика мембраны эритроцита и ее изменения при патологиях разного генеза. Бюл. ВСНЦ СО РАМН. № 3. С. 334. (Borovskaya M.K., Kuznetsova E.E., Gorokhova V.G., Koriakina L.B., Kurilskaya T.E., Pivovarov Ju.I. 2010. Structural and functional characteristics of membrane’s erythrocyte and its change at pathologies of various genesis. Byulleten’ Vostochno-Sibirskogo Nauchnogo Tsentra Sibirskogo Otdeleniya Rossiiskoi Akademii Meditsinskikh Nauk. № 3. P. 334.)

2. Боронихина Т.В., Ломановская Т.А., Яцковский А.Н. 2021. Плазмолемма эритроцитов и ее изменения в течение жизни клеток. Журн. анатомии и гистопатологии. Т. 10. № 2. С. 62. (Boronikhina T.V., Lamanovskaya T.A., Yatskovskii A.N. 2021. Erythrocyte plasmalemma and its changes during the cell lifespan. Zhurnal Anatomii i Gistopatologii. V. 10. № 2. P. 62.)

3. Иванова В.П. 2023. О вариативности клеточного адгезивного ответа под воздействием родственных коротких пептидов. Цитология. Т. 65. № 1. С. 92. (Ivanova V.P. 2023. On the variativity of cellular adhesive response under the influence of related short peptides. Cell Tissue Biol. (Tsitologiya). V. 17. № 3. P. 265.)

4. Иванова В.П., Гринчук Т.М., Алексеенко Л.Л., Арцыбашева И.В., Гаврилова И.И. 2010. Влияние синтетического поликатиона полиаллиламина на адгезию и жизнеспособность фибробластов китайского хомячка CHL V-79 RJK с разной степенью устойчивости к нагреву. Цитология. Т. 52. № 9. С. 729. (Ivanova V.P., Grinchuk T.M., Alekseenko L.L., Artsybasheva I.V., Gavrilova I.I. 2010. Effect of synthetic polycation polyallylamine on adhesion and viability of CHL V-79 RJK Chinese hamster fibroblasts with various heat resistance. Cell Tiss. Biol. V. 4. P. 520.)

5. Иванова В.П., Ковалева З.В., Анохина В.В., Кривченко А.И. 2012. Влияние трипептидного фрагмента коллагена (GER) на адгезию и распластывание фибробластов зависит от свойств адгезивной поверхности. Цитология. Т. 54. № 11. С. 823. (Ivanova V.P., Kovaleva Z.V., Anokhina V.V., Krivchenko A.I. 2013. The effect of a collagen tripeptide fragment (GER) on fibroblast adhesion and spreading depends on properties of an adhesive surface. Cell Tiss. Biol. V. 7. P. 21.)