PARAMETERS OF ENERGY METABOLISM AND ADENYLATE SYSTEM OF MYTILUS GALLOPROVINCIALIS TISSUES (LAMARCK. 1819) IN CONDITIONS OF MODERATE HYPOXIA

Abstract

The effect of moderate hypoxia on the processes of energy metabolism in the tissues (gills, hepatopancreas) of the Mediterranean mussel (Mytilus galloprovincialis, Lamarck, 1819) was studied experimentally. The control group of mollusks contained 6.8–6.9 mgO2 l–1, the experimental group at 1.9–2.0 mgO2 l–1. In both cases, the water temperature was 22 ± 1°C, the salinity was 17–18‰. The exposition – 72 hours. The oxygen content in the water was lowered by bubbling with nitrogen gas for 4–5 hours. In conditions of moderate hypoxia, a complex of reactions aimed at maintaining the energy status of tissues developed in the body of the Mediterranean mussel. Aerobic processes were clearly limited, as evidenced by a decrease in succinate dehydrogenase (SDH) activity. At the same time, the processes of anaerobic glycolysis intensified. The activity of aldolase, malate dehydrogenase (MDH) increased, the content of pyruvate in tissues increased. This was not accompanied by an increase in lactate dehydrogenase (LDH) activity and an increase in lactate content. The tissues retained their original energy status. The content of the ATP fraction remained at the level of control values, which reflected the adaptive orientation of the reorganization of tissue metabolism. The ability of hepatopancreas to accumulate ADP and AMP fractions from circulation systems under experimental hypoxic load was noted.