Expression of muscle-specific genes in atlantic salmon fingerlings (SALMO SALAR L.) in the conditions of aquaculture under the influence of different lighting and feeding regimes

Abstract

The expression level of myosin heavy and light chains genes (myhc, mlc-2), transcription myogenic regulation factors (myf5, myog, myod1 paralogs), myostatin paralogs and insulin-like growth factors (igf1 and igf2) in the muscles of salmon fingerlings artificially grown under different lighting and feeding conditions at a constant water temperature was studied in the region of North Ossetia-Alania. The combined effect of lighting and feeding modes was reflected in the expression of the genes myhc, mlc-2, myod1a, myf5, myod1b, myod1c. Thus, individuals reared under constant lighting conditions were distinguished by higher levels of expression of the myf5, myhc and mlc-2 genes compared to fish in natural light, which also corresponded to their higher growth rates. The expression of IGF genes varied in different ways throughout the experiment: the expression level of the igf1 gene increased, and the igf2 gene first increased after a month of study, then decreased on the second month. The results indicate differences in the processes of myogenesis regulation depending on lighting and feeding conditions.