EFFECTS OF COLD SHOCK ON THE SIZE AND ACTIVITY OF SCORPAENA PORCUS (LINNAEUS, 1758) NUCLEAR ERYTHROCYTES (IN VITRO EXPERIMENTS)-Reference-Cited by-同舟云学术

EFFECTS OF COLD SHOCK ON THE SIZE AND ACTIVITY OF SCORPAENA PORCUS (LINNAEUS, 1758) NUCLEAR ERYTHROCYTES (IN VITRO EXPERIMENTS)

Published:2023-05-01 Issue:3 Volume:59 Page:168-177
ISSN:0044-4529
Container-title:Журнал эволюционной биохимии и физиологии
language:
Short-container-title:Žurnal èvolûcionnoj biohimii i fiziologii

Author:

Shalagina N. E.¹,Soldatov A. A.¹,Bogdanovich Yu. V.¹

Affiliation:

1. Kovalevsky Institute of Biology of the Southern Seas, RAS

Abstract

The in vitro effect of cold shock on erythrocytes of the benthic scorpionfish Scorpaena porcus L. was studied. The blood samples were exposed to 22°С (control group) and 4°С (experimental group) for 3 hours. Cold shock had no significant impact on erythrocyte scorpionfish vital function. The cells maintained the integrity of cytoplasmic membranes, mitochondrial potential and the level of oxidative processes, that the constant values of Propidium Iodide (PI), Rhodamine 123 (R123) and 2',7'-Dichlorodihydrofluorescein diacetate (DCF-DA) fluorescence levels demonstrated. Main changes referred to erythrocyte shape and nucleus functional activity. Cells gained more roundish shape, nucleus size increased with the growth of the nuclear-cytoplasmic ratio (NCR) and the decrease of fluorescence intensity of the cells labeled with SYBR Green I. It appears to be related to the activation of transcriptomic processes.

Publisher

The Russian Academy of Sciences

Reference50 articles.

1. Donaldson MR, Cooke SJ, Patterson DA, Macdonald JS (2008) Cold shock and fish. J Fish Biol 73: 1491–1530. https://doi.org/10.1111/j.1095-8649.2008.02061.x

2. Reid CH, Patrick PH, Rytwinski T, Taylor JJ, Willmor WG, Reesor B, Cooke SJ (2022) An updated review of cold shock and cold stress in fish. J Fish Biol 100: 1102–1137. https://doi.org/10.1111/jfb.15037

3. Koakoski G, Oliveira TA, da Rosa JGS, Fagundes M, Kreutz LC, Barcellos LJG (2012) Divergent time course of cortisol response to stress in fish of different ages. Physiol Behav 106 (2): 129–132. https://doi.org/10.1016/j.physbeh.2012.01.013

4. He J, Qiang J, Yang H, Xu P, Zhu ZX, Yang RQ (2015) Changes in the fatty acid composition and regulation of antioxidant enzymes and physiology of juvenile genetically improved farmed tilapia Oreochromis niloticus (L.), subjected to short-term low temperature stress. J Thermal Biol 53:90–97. https://doi.org/10.1016/j.jtherbio.2015.08.010

5. Bai Y, Liu H, Huang B, Wagle M, Guo S (2016) Identification of environmental stressors and validation of light preference as a measure of anxiety in larval zebrafish. BMC Neurosci 17 (1): 1–12. https://doi.org/10.1186/s12868-016-0298-z

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