Resins Based on Polystyrene–Divinylbenzene with Attached Hydrophilized Polyethyleneimine for Ion and Hydrophilic Interaction Liquid Chromatography

Abstract

To expand the field of application of anion exchangers based on a copolymer of styrene and divinylbenzene with attached polyethyleneimine, quaternized with glycidol, the following conditions for their synthesis were varied: the amount of the added glycidol, temperature, and duration of synthesis. The influence of these factors on the capacity, selectivity, and efficiency of the resins in the mode of suppressed ion chromatography was studied; in addition, the stationary phases were studied in the mode of hydrophilic interaction liquid chromatography using the Tanaka test. It was shown that the synthesis conditions under study ensure the control of the capacity and selectivity of anion exchangers, while their hydrophilicity changes insignificantly. The behavior of oxoanions on covalently attached resins in the ion chromatography mode was studied for the first time, and the applicability of phases with quaternized polyethyleneimine in the hydrophilic interaction liquid chromatography mode to the separation of sugars, amino acids, water-soluble vitamins, nucleosides, and nitrogenous bases was demonstrated for the first time.