Effect of <i>n</i>-acetylcysteine on cu²⁺(fe²⁺)/h₂o₂-mediated free radical fragmentation of glycerophosphate in the presence of biologically active compounds

Abstract

The effect of N -acetylcysteine on homolytic fragmentation of β-glycerophosphate with cleavage of phosphoester bond was studied. It was shown that, N -acetylcysteine activates the fragmentation induced by Cu2+(Fe2+)-H2O2, and inhibits the radiation-induced process. The stimulated effect of N -acetylcysteine on Cu2+/H2O2-mediated fragmentation in the presence of compounds affecting on HO• level, the Cu2+/Cu+ ratio, or interacting with thiol is either enhanced (Trolox, Ubiquinone Q0) or switched to a prоtective one (NaN3, Met, ascorbate). In the presence of Fe2+ Trolox unlike Q0 reduces the promoter effect of N -acetylcysteine.