Safety assessment of Bаcillus atrophaeus В-11474, which is promising for the development of a microbial biological product with complex action

Abstract

Representatives of the genus Bacillus are known as producers of a wide range of antagonist compounds of different structures (bacteriocins, antimicrobial peptides and lipopeptides, polyketides and siderophores) with growth-stimulating and protective properties. A necessary condition for the use of microbial objects in biotechnological processes is safety for human and animal health. The safety of Bacillus atrophaeus B-11474 strain, which is the basis of a microbial biopreparation of complex action to increase the yield and protect agricultural plants from diseases according to the indicators of virulence, acute toxicity, toxigenicity and ability to dissemination was studied.The non-pathogenicity of Bacillus atrophaeus strain B-11474 was established by testing on white Balb/c mice. The studied strain was deposited in the All-Russian Collection of Industrial Microorganisms (VKPM). The experiment was performed in compliance with all established regulatory requirements for work with laboratory animals according to GLP rules and ethical ethical standards for handling animals. The results of investigation of Bacillus atrophaeus VKPM B-11474 live culture suspension revealed no adverse effect on the experimental animals, indicating the absence of virulence, acute toxicity, toxigenicity and dissemination. Mice actively consumed food and water, were motile, with adequate response to experimenters and physical factors, no lethal outcome was observed. The autopsy has shown that the surface of the internal organs was smooth, without visible pathology, with normal color and of dense structure. The test culture was not found in smears-imprints of internal organs. It was shown that Bacillus atrophaeus strain VKPM B-11474 is promising for obtaining a biopreparation safe for use in agriculture and can be used for further research.