Decomposition of Small-Angle Scattering Profiles from Two Conformational States of 3-Isopropylmalate Dehydrogenase Using Evolving Factor Analysis-Reference-Cited by-同舟云学术

Decomposition of Small-Angle Scattering Profiles from Two Conformational States of 3-Isopropylmalate Dehydrogenase Using Evolving Factor Analysis

Published:2023-11-01 Issue:6 Volume:68 Page:946-950
ISSN:0023-4761
Container-title:Кристаллография
language:
Short-container-title:Kristallografiâ

Author:

Konarev P. V.¹²,Volkov V. V.¹³

Affiliation:

1. Shubnikov Institute of Crystallography, Federal Scientific Research Centre “Crystallography and Photonics,” Russian Academy of Sciences, 119333, Moscow, Russia

2. National Research Centre “Kurchatov Institute”, 123182, Moscow, Russia

3. National Research Centre “Kurchatov Institute,”, 123182, Moscow, Russia

Abstract

The separation of two conformational states of 3-isopropylmalate dehydrogenase molecules from Thermus thermophilus in solution on a gel chromatographic column, attached to a sample cell of a small-angle X-ray scattering synchrotron beamline, has been simulated. The scattering intensity profiles from the open and closed forms of the enzyme molecules were restored by evolving factor analysis (EFA) using the synthetic data set with added Poisson noise at the relative level of 3–5%. Thus, the efficiency of the EFA algorithm is confirmed in the case of two-component mixtures consisting of particles with the same molecular masses.

Publisher

The Russian Academy of Sciences

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