Latex Agglutination as an Alternative to Hemagglutination Reaction of Influenza Viruses-Reference-Cited by-同舟云学术

Latex Agglutination as an Alternative to Hemagglutination Reaction of Influenza Viruses

Published:2023-09-01 Issue:5 Volume:57 Page:898-906
ISSN:0026-8984
Container-title:Молекулярная биология
language:
Short-container-title:Molekulârnaâ biologiâ

Author:

Ivanov P. A.¹²,Lyashko A. V.¹,Ionov S. A.¹³,Shcherbinin D. N.¹,Rudneva I. A.¹,Shilov A. A.¹,Bunkova N. I.¹,Shmarov M. M.¹⁴,Timofeeva T. A.¹

Affiliation:

1. National Research Centre for Epidemiology and Microbiology named after the honorary academician N.F. Gamaleya, Ministry of Health of the Russian Federation

2. Winogradsky Institute of Microbiology RC Biotechnology, Russian Academy of Sciences

3. Mendeleev University of Chemical Technology

4. Sechenov First Moscow State University, Ministry of Health of the Russian Federation

Abstract

As an alternative to the classical method of erythrocyte hemagglutination, a latex agglutination assay based on the interaction of influenza viruses with the sialoglycoprotein fetuin immobilized on the surface of polystyrene microspheres, has been developed. Twelve influenza A virus strains of different subtypes and two influenza B viruses of different lines were tested. Simultaneous titration of viruses using the classical hemagglutination test and the proposed latex agglutination assay showed similar sensitivity and a high degree of correlation (R = 0.94). The obtained microspheres can be used for titration of viruses that recognize and bind sialylated glycans as receptors. In particular, latex aggregation was also induced by the Newcastle disease virus.

Publisher

The Russian Academy of Sciences

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