Identification of Clinical Isolates of <i>Bacillus cereus</i> Group and Their Characterization by Mass Spectrometry and Electron Microscopy-Reference-Cited by-同舟云学术

Identification of Clinical Isolates of <i>Bacillus cereus</i> Group and Their Characterization by Mass Spectrometry and Electron Microscopy

Published:2023-07-01 Issue:4 Volume:57 Page:609-622
ISSN:0026-8984
Container-title:Молекулярная биология
language:
Short-container-title:Molekulârnaâ biologiâ

Author:

Smirnova T. A.¹,Polyakov N. B.¹²,Karpov D. S.³,Solovyev A. I.¹,Shevlyagina N. V.¹,Andreevskaya S. G.¹,Shcherbinin D. N.¹,Plieva Z. S.¹,Kozlova V. A.¹,Pereborova A. A.¹,Bogdanov I. A.¹⁴,Grumov D. A.¹,Zubasheva M. V.¹,Poddubko S. V.⁵,Grechnikov A. A.²,Sukhina M. A.⁶,Zhukhovitsky V. G.¹⁷

Affiliation:

1. Gamaleya National Research Centre for Epidemiology and Microbiology, Ministry of Public Health of the Russian Federation

2. Vernadsky Institute of Geochemistry and Analytical Chemistry, Russian Academy of Sciences

3. Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Engelhardt Institute of Molecular Biology, Russian Academy of Sciences

4. “Nanopromimport” LLC, Moscow State University Science Park

5. State Scientific Center of the Russian Federation, Institute of Bio-Medical Problems, Russian Academy of Sciences

6. Ryzhikh State Research Center of Coloproctology, Ministry of Health

7. Russian Medical Academy of Continuing Professional Education (RMANPO), Ministry of Health of the Russian Federation

Abstract

Bacillus cereus is a spore-forming bacterium found in the environment mainly in soil. Bacillus spores are known to be extremely resistant not only to environmental factors, but also to various sanitation regimes. This leads to spore contamination of toxin-producing strains in hospital and food equipment and, therefore, poses a great threat to human health. Two clinical isolates identified as B. cereus and B. cytotoxicus were investigated in the present work. It was shown that their calcium ion content was significantly lower than that of the reference strains. According to electron microscopy, one of the isolates SRCC 19/16 has an enlarged exosporium, and isolate SRCC 1208 shows large electron-dense inclusions of unclear nature during sporulation. We can assume that it contains a biologically active component with a cytotoxic effect and possibly plays a role in pathogenesis. Comparative chemical, biochemical, physiological, and ultrastructural analysis of spores of clinical isolates and reference strains of B. cereus was performed. The results obtained deepen our understanding of the properties of spores that contribute to the increased pathogenicity of B. cereus group species.

Publisher

The Russian Academy of Sciences

Reference38 articles.

1. Savini V. (2016) In: The Diverse Faces of Bacillus cereus. Ed. Savini V. Elsvier Acad. Press, pp. 73–84.

2. Kuroki R., Kawakami K., Qin L., Kaji C., Watanabe K., Kimura Y., Ishiguro C., Tanimura S., Tsuchiya Y., Hamaguchi I., Sakakura M., Sakabe S., Tsuji K., Inoue M., Watanabe H. (2009) Nosocomial bacteremia caused by biofilm-forming Bacillus cereus and Bacillus thuringiensis. Intern. Med. 48(10), 791–796.

3. Elshaghabee F.M.F., Rokana N., Gulhane R.D., Sharma C., Panwar H. (2017) Bacillus as potential probiotics: status, concerns, and future perspectives. Front. Microbiol. 8, 1490.

4. Bundy J.G., Willey T.L., Castell R.S., Ellar D.J., Brindle K.M. (2005) Discrimination of pathogenic clinical isolates and laboratory strains of Bacillus cereus by NMR-based metabolomic profiling. FEMS Microbiol. Lett. 242(1), 127–136.

5. Smirnova T.A., Zubasheva M.V., Shevlyagina N.V., Nikolaenko M.A., Azizbekyan R.R. (2013) Electron microscopy of the surfaces of Bacillus spores. Microbiology. 82(6), 713–720.