DNA Fragment Enrichment for High-Throughput Sequencing-Reference-Cited by-同舟云学术

DNA Fragment Enrichment for High-Throughput Sequencing

Published:2023-05-01 Issue:3 Volume:57 Page:440-457
ISSN:0026-8984
Container-title:Молекулярная биология
language:
Short-container-title:Molekulârnaâ biologiâ

Author:

Sinyakov A. N.¹,Kostina E. V.¹

Affiliation:

1. Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences

Abstract

Application of oligonucleotides, mainly obtained using new generation DNA synthesizers (microarray DNA synthesizers), for the enrichment of targeted genomic fragments are described. Methods of molecular hybridization, polymerase chain reaction and CRISPR-based methods for targets enrichment are considered. Examples of the practical use of the developed methods for research and diagnostic purposes are given.

Publisher

The Russian Academy of Sciences

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