An <i>in vitro Arabidopsis thaliana</i> Root Structural and Functional Characterization

Author:

Bulavin I. V.1,Sidyakin A. I.12

Affiliation:

1. Federal State Funded Institution of Science “The Labor Red Banner Order Nikita Botanical Gardens – National Scientific Center of the RAS”

2. Institute of Biochemical Technologies, Ecology and Pharmacy of Federal State Autonomous Educational Institution of Higher Education “V.I. Vernadsky Crimean Federal University”

Abstract

Two models of Arabidopsis thaliana rhizogenesis in vitro were studied: 1) from callus and 2) leaf explants petioles on the Murashige and Skoog one-tenth strength hormone-free nutrient medium and also with the addition of the growth regulator such as indole-3-butyric acid. Morphological and anatomical studies show significant changes in the structure of the roots formed de novo in vitro from callus tissue, while the organs from leaf explant petioles were similar to those formed from the seed (primary). By Sabinin-Kolosov method, a decrease in the percentage of active root surface was established. Occurrence of the structural changes during in vitro rhizogenesis and their effect on root functionality are discussed.

Publisher

The Russian Academy of Sciences

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