Affiliation:
1. Vitebsk State Order of Peoples’ Friendship Medical University
2. Belarussian State Medical University
Abstract
TNFRSF12AmRNA expression and distribution of TNFRSF12A+ cells were studied in detail for the first time at different stages of fibrosis in rat liver. Under physiological conditions, the expression level ofTNFRSF12AmRNA was 0.224 (95% CI: 0.170–0.277). At the same time, cells expressing theTNFRSF12Amarker were practically absent. In bridging fibrosis, the first peak ofTNFRSF12AmRNA growth (p=0.000) and an increase in the area of TNFRSF12A+ cells (p=0.000) was established. The second peak (p=0.000) was detected during the process of transformation of fibrosis into cirrhosis. At the stage of incomplete cirrhosis, a sharp drop was noted. A subsequent increase in the expression ofTNFRSF12AmRNA and the area of TNFRSF12A+ cells was observed from the stage of significant cirrhosis. Immunohistochemical method revealed two groups of TNFRSF12A+ cells. In the sinusoidal capillaries TNFRSF12A+, the cells had a shape close to flat and resembled endotheliocytes, while in the fibrous connective tissue they were rounded. The number ofα-SMA+ cells increased gradually (p=0.000) before the onset of significant cirrhosis, and then there was a sharp increase (p=0.000).
Publisher
The Russian Academy of Sciences
Reference30 articles.
1. Банин В.В., Быков В.Л. Международные термины по цитологии и гистологии человека с официальным списком русских эквивалентов. М.: ГЭОТАР-Медиа, 2009. 272 с.
2. Коржевский Д.Э. Теоретические основы и практическое применение методов иммуногистохимии. СПб.: СпецЛит, 2014. 119 с.
3. Жижин К.С. Медицинская статистика: учебное пособие. Ростов н/Д: Феникс, 2007. 160 с.
4. Breit H.C., Block K.T., Winkel D.J., Gehweiler J.E., Henkel M.J., Weikert T., Stieltjes B., Boll D.T., Heye T.J. Evaluation of liver fibrosis and cirrhosis on the basis of quantitative T1 mapping: Are acute inflammation, age and liver volume confounding factors? // Eur. J. Radiol. 2021. № 141. Р. 109789. https://doi.org/10.1016/j.ejrad.2021.109789
5. Burkly L.C., Michaelson J.S., Hahm K., Jakubowski A., Zheng T.S. TWEAKing tissue remodeling by a multifunctional cytokine: role of TWEAK/Fn14 pathway in health and disease // Cytokine. 2007. V. 40. № 1. Р. 1–16. https://doi.org/10.1016/j.cyto.2007.09.007