<i>Aspergillus tabacinus</i> as a producer of antithrombotic proteases

Abstract

Microfungi of the genus Aspergillus are well-known as producers of fibrinolytic and plasminogen activating proteases. But for development of new antithrombotics we should use strains which extracellular proteases correspond to these criteria: 1) demonstrate anticoagulant, fibrinolytic and plasminogen activating activities at the same time; 2) have narrow substrate specificity; 3) are able to hydrolase substrates of two following each other proteins of hemostasis system. According to these criteria Aspergillus tabacinus was chosen. Maximal activities of culture liquid of this strain grown in optimal conditions were 87 Е × 10–3 with activated protein C substrate S-2366 and 73 Е × 10–3 with thrombin substrate Chromozym TH. Fibrinogenolytic activity of lyophilized enzyme preparation after ammonium sulfate precipitation and dialysis was 779.1 Е/mg of protein.