Affiliation:
1. Institute of Biochemistry and Physiology of Plants and Microorganisms, Saratov Scientific Center,
Russian Academy of Sciences
Abstract
Abstract—Inactivation of one of the genes (CDS AZOBR_p60123) of the ABC transporter ATP-binding protein Wzt in the p60123::aphAI mutant of Azospirillum baldaniorum Sp245 and the introduction of an additional copy of this gene into the parent or mutant strain affected the chain-length heterogeneity of O polysaccharides (OPSs) and lipopolysaccharides (LPSs), the OPS and LPS immunochemical characteristics, and the strain properties related to polysaccharide production (biofilm construction). Biofilms of the p60123::aphAI mutant Sp245.4-1-1 accumulated two times less biomass than those of Sp245. Introduction of pRK415-p60123 into the cells of Sp245.4-1-1 or Sp245 resulted in a respective increase or decrease in the ability of the resulting derivative strains to accumulate biofilm biomass, as compared with the original strains.
Publisher
The Russian Academy of Sciences
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