Hydrogels based on recombinant spidroin stimulate proliferation and migration of human cornea cells-Reference-Cited by-同舟云学术

Hydrogels based on recombinant spidroin stimulate proliferation and migration of human cornea cells

Published:2024-04-15 Issue:2 Volume:515 Page:49-54
ISSN:2686-7389
Container-title:Доклады Российской академии наук. Науки о жизни
language:
Short-container-title:Doklady Rossijskoj akademii nauk. Nauki o žizni

Author:

Agapova O. I.¹,Ostrovsky D. S.²,Khubetsova M. Kh.²,Kerimov T. Z.²³,Borzenok S. A.²³,Bogush V. G.⁴,Davydova L. I.⁴,Cheperegin S. E.⁴,Efimov A. E.¹,Agapov I. I.¹,Debabov V. G.⁴

Affiliation:

1. Academician V.I.Shumakov Federal Research Center of Transplantology and Artificial Organs of the Ministry of Healthcare of the Russian Federation

2. S. Fyodorov Eye Microsurgery Complex Federal State Institution of the Ministry of Healthcare of the Russian Federation

3. A.I. Evdokimov Moscow State University of Medicine and Dentistry of the Ministry of Healthcare of the Russian Federation

4. The National Research Centre “Kurchatov Institute”

Abstract

This article presents the results of studying the impact of recombinant spidroin hydrogel on posterior epithelial cells and human corneal keratocytes in an in vitro experiment. The World Health Organization in its studies has established a high prevalence of corneal injuries among the population of developing countries. In recent years, various technologies have been proposed to restore the damaged surface of the cornea. The use of biodegradable silk-based materials, such as spidroins is one of the main parts of scientific research of corneal regeneration. Spidroinsare well known for their optimal balance of strength and elasticity, which, given their biological compatibility, non-immunogenicity and biodegradability, allows them to be used as a biomaterial for tissue engineering and regenerative medicine. In this reason a detailed assessment of the cytotoxicity of hydrogels based on recombinant rS2/12-RGDS spidroinon the epithelial cells and keratocytes was performed here, taking into attention possible changes of the phenotype and migratory activity of these cells. This study demonstrates the promise and therapeutic potential of hydrogels based on recombinant spidroin.

Publisher

The Russian Academy of Sciences

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