Identification of ultrastructural details of the astrocyte process system in neural tissue of the brain using correlative scanning probe and transmission electron microscopy-Reference-Cited by-同舟云学术

Identification of ultrastructural details of the astrocyte process system in neural tissue of the brain using correlative scanning probe and transmission electron microscopy

Published:2024-04-15 Issue:2 Volume:515 Page:76-80
ISSN:2686-7389
Container-title:Доклады Российской академии наук. Науки о жизни
language:
Short-container-title:Doklady Rossijskoj akademii nauk. Nauki o žizni

Author:

Agapova О. I.¹,Efimov А. Е.¹,Obraztsova E. A.²³,Mochalov K. E.²,Solovyeva D. O.²,Oleinikov V. A.²⁴,Agapov I. I.¹,Gautier S. V.¹⁵

Affiliation:

1. Shumakov National Medical Research Center of Transplantology and Artificial Organs

2. Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences

3. Moscow Institute of Physics and Technology

4. National Research Nuclear University MEPhI (Moscow Engineering Physics Institute)

5. Sechenov University

Abstract

Nanoscale morphological features of branched processes of glial cells may be of decisive importance for neuron-astrocytic interactions in health and disease. The paper presents the results of a correlation analysis of images of thin processes of astrocytes in the nervous tissue of the mouse brain, obtained by scanning probe microscopy and transmission electron microscopy with high spatial resolution. Samples were prepared and imaged using a unique hardware combination of ultramicrotomy and scanning probe microscopy. It was shown that the images identified details of astrocytes with a thickness of the order of tens of nanometers, which can be used in the future to reconstruct the three-dimensional structure of astrocytic processes by integrating a series of sequential images of ultrathin sections of nervous tissue.

Publisher

The Russian Academy of Sciences

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