Enhancing human glycoprotein hormones production in CHO cells using heterologous beta-chain signal peptides-Reference-Cited by-同舟云学术

Enhancing human glycoprotein hormones production in CHO cells using heterologous beta-chain signal peptides

Published:2024-07-05 Issue:1 Volume:514 Page:31-35
ISSN:2686-7389
Container-title:Доклады Российской академии наук. Науки о жизни
language:
Short-container-title:Doklady Rossijskoj akademii nauk. Nauki o žizni

Author:

Sinegubova M. V.¹,Kolesov D. E.¹,Dayanova L. K.¹,Vorobiev I. I.¹,Orlova N. A.¹

Affiliation:

1. The Federal State Institution “Federal Research Centre “Fundamentals of Biotechnology” of the Russian Academy of Sciences”

Abstract

We studied the influence of heterologous signal peptides in the β-chains of glycoprotein hormones on the biosynthesis of these hormones in a transiently transfected culture of Chinese hamster ovary cells CHO S. When replacing the natural signal peptides of the β-chains with the heterologous signal peptide of human serum albumin, cell productivity was increased by 2–2.5 times for human luteinizing hormone, human chorionic gonadotropin, human thyroid-stimulating hormone, but not for human follicle-stimulating hormone. No significant increase in cell productivity was observed for human azurocidin signal peptide and human glycoprotein hormone α-chain signal peptide. The used approach allows quick assessing the effect of heterologous signal peptides on the biosynthesis of heterodimeric proteins of various classes.

Publisher

The Russian Academy of Sciences

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