Activation of embryonic gene transcription in neural precursor cells derived from induced pluripotent stem cells of patients with Parkinson’s disease

Author:

Fedoseyeva V. B1,Novosadova E. V1,Nenasheva V. V1,Novosadova L. V1,Grivennikov I. A1,Tarantul V. Z1

Affiliation:

1. National Research Center “Kurchatov Institute”

Abstract

Parkinson’s disease (PD) is one of the most common neurodegenerative diseases in the world. Despite numerous studies, the causes of this pathology remain completely unknown. This is, among other things, due to the difficulty of obtaining biological material for analysis. Neural cell cultures derived from induced pluripotent stem cells (iPSCs) serve as a great potential for studying the molecular events underlying the pathogenesis of PD. This paper presents the results of bioinformatic analysis of data obtained using RNA-seq technology in the study of neural precursors (NP) obtained from iPSCs of healthy donors and patients with PD carrying various mutations that are a common cause of familial PD. This analysis showed that in NP cells obtained from PD patients, unlike healthy donors, the level of transcription of genes actively expressed in the nervous system at the embryonic stage of development was significantly increased. Bioinformatic data are generally confirmed by real-time PCR. The data obtained suggest that one of the causes of PD may be an abnormal process of enhancing the expression of embryonic genes during differentiation of neural cells (dematuration).

Publisher

The Russian Academy of Sciences

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