Effect of Glucose on Water Transport in Rat Peritoneal Mesothelium Cells

Abstract

Glucose is widely used as an osmotic agent in the preparation of solutions for peritoneal dialysis. The aim of this work was to study the effect of glucose on the expression of the water channel aquaporin- 1 (AQP1) and the transcription factor TonEBP, as well as on water permeability of cells in the primary mesothelial mesentery culture of Wistar rats. The method of fluorescence microscopy with intracellular dye сalcein was used in the work. The mRNA content was evaluated by real time RT-PCR. Preincubation of cells in a DMEM medium containing glucose (2.3%, 24 hours, 37°C, 5% CO2) led to a decrease in the expression of the aqp1 gene by approx. 60% (control 0.87 ± 0.18; glucose 0.34 ± 0.12, n = 9, p 0.05) and tonebp by 20% (control 0.37 ± 0.024; glucose 0.3 ± 0.012, n = 9, p 0.05). A decrease by 40% in the permeability to water of the plasma membrane of peritoneal mesothelium cells after 24 h preincubation with glucose was also observed (control 7.3Е–3 ± 1.3Е–3 (sm/s), n = 27; glucose 4.3E–3 ± ± 3.8E–4 (sm/s), n = 57, p 0.01). The results of the study indicate that glucose can have an inhibitory effec-t on the transport processes in the cells of the peritoneal mesothelium, which, with multiple exposures, can lead to structural and functional disorders of the peritoneal membrane.