Affiliation:
1. Nakhchivan State University
2. Academician Abdulla Garayev Institute of Physiology, NAS
Abstract
The goal of the study was analysis of the role for dihydropyrimidinase-related protein 2 (DRP2) in regulation of anxiety in humans and animals. For this purpose, the levels of DRP2 in the platelets and saliva and the levels of natural autoantibodies to DRP2 in the blood serum of the patients, admitted for a surgical operation, were analyzed alongside with the effects of DRP2 on rat behavior using the model of elevated plus-maze. Blood samples were collected from the veins of the patients, feeling anxiety at the day of surgery, into test tubes containing 5% EDTA as an anticoagulant. Platelets and serum were purified and through indirect ELISA-test the levels of DRP2 were evaluated in the platelets, while the levels of natural anti-DRP2 autoantibodies were measured in the blood serum. Concomitantly, the levels of DRP2 were estimated in the patients’ saliva sampled on the same day. The results showed upregulation of DRP2 in the platelets (p 0.05 on Student’s t-criterion) and natural anti-DRP2 autoantibodies in the serum of the patients (p 0.001) relative to healthy persons. Evaluation of the level of the stress hormone cortisol in the patients’ serum revealed its upregulation (p 0.01). At the same time downregulation of DRP2 in the patients’ saliva (p 0.01) was noticed. Intra-cerebral administration of DRP2 to the rats resulted in an increase in the number of crossed squares in the open arms of the maze relative to the control rats injected with inactivated DRP2 (p 0.01, Wilcoxon and Mann–Whitney U-criterion) which indicates their reduced anxiety. Based on the data obtained we conclude that evaluation of DRP2 in the platelets, natural anti-DRP2 autoantibodies in the blood serum and DRP2 in the saliva might serve as an indicator of anxiety levels in humans.
Publisher
The Russian Academy of Sciences
Reference17 articles.
1. Shapell S, Detwiler C, Boquet A, Wiegmann D (2006) Human error and commercial aviation accidents: A comprehensive, fine-grained analysis using HFACS. Federal Aviation Administration. DOT/FAA/AM-06/18. Office of Aerospace Medicine Washington, DC 20591.
2. Devido G, Jones M, Geraci M, Hollon N, Blair RJ, Pine DS, Blair K (2009) Stimulus-reinforcement-based decision making and anxiety: impairment in generalized anxiety disorder (GAD) but not in generalized social phobia (GSP). Psychol Med 39: 1153–1161. https://doi.org/10.1017/S003329170800487X
3. Luhman CC, Ishida K, Hajcak G (2011) Intolerance of uncertainty and decisions about delayed, probabilistic rewards. Behav Ther 42: 378–386. https://doi.org/10.1016/j.beth.2010.09.002
4. Teng C, Otero M, Geraci M, Blair RJR, Pine DS, Grillon Ch, Blair KS (2016) Abnormal decision-making in generalized anxiety disorder: Aversion of risk or stimulus-reinforcement impairment? Psych Res 237: 351–356. https://doi.org/10.1016/j.psychres.2015.12.031
5. Elliott JM, Kent A (1989) Comparison of [125I]iodolysergic acid diethylamide binding to human frontal cortex and platelet tissue. J Neurochem 53: 191–196. https://doi.org/10.1111/j.1471-4159.1989.tb07313.x