Affiliation:
1. Institute of Molecular Biology and Medicine
2. Skryabin Kyrgyz National Agrarian University
3. Institute of Genetics and Cytology of the National Academy of Sciences of Belarus
Abstract
The aim was to ascertain genetic structure and diversity of Kyrgyz mountainous merino via microsatellite DNA markers. We analyzed DNA samples of 109 Kyrgyz mountaneous merino specimens, bred in three state breeding factories (STB), including “Orgochor” in the Issykul Province, “Katta-Taldyk” in the Osh Province and STB named after Luschikhin in the Talas Province. We identified 126 alleles in 12 microsatellite loci (McM042, INRA006, McM527, ETH152, CSRD247, OarFCB20, INRA172, INRA063, MAF065, MAF214, INRA005, INRA023). There were 6 to 16 alleles in each locus (mean 10.500 ± 0.957 alleles per locus). We identified 67 rare alleles (prevalence less than 5.0%), which made 53.2% of all alleles found. The greatest number of alleles were found in INRA023 (12 alleles), INRA005 (13 alleles), OarFCB20 and INRA063 (14 in each) and CSRD247 (16 alleles) autosomal loci. Based on the mean number per locus (Na = = 10.500 ± 0.957), number of efficient alleles (Ne = 4.556 ± 0.394), the observed (Ho = 0.731 ± 0.023) an expected (He = 0.761 ± 0.021) heterozygosity, we conclude high genetic diversity of the selected sheep sample attributed to Kyrgyz mountainous merino and its significant genetic potential.
Publisher
The Russian Academy of Sciences