Affiliation:
1. State Forensic Examination Committee of the Republic of Belarus
2. Scientific and Practical Center of the State Forensic Examination Committee of the Republic Belarus
3. RUP “BelJurZabespjachjenne”
Abstract
To establish a system for DNA identification of biological samples of the raccoon dog (Nyctereutes procyonoides), we carried out a study of polymorphism of 39 microsatellite loci and three sex loci specific to various species of the Canidae family. This resulted in the development of the NPlex test system, which includes 14 autosomal STRs and two sex loci. The test system has been validated for use in forensic identification of racoon dogs based on biological traces (such as traces of blood and secretions, fragments of muscle tissue or bones, dermis and hair, etc.) found at crime scenes, as well as for establishing biological relationship of animals of this species. Validation efforts were performed in accordance with the protocol of the Scientific Working Group on DNA Analysis Methods.
Publisher
The Russian Academy of Sciences
Reference28 articles.
1. Yan S.Q., Li Y.M., Bai C.Y. et al. Development and characterization of polymorphic microsatellite markers for Chinese raccoon dog (Nyctereutes procyonoides procyonoides) // Genet. Mol. Res. 2013. V. 12. P. 6351–6355. https://doi.org/10.4238/2013.December.6.2
2. Hong Y., Kim K.S., Lee H., Min M.S. Population genetic study of the raccoon dog (Nyctereutes procyonoides) in South Korea using newly developed 12 microsatellite markers // Genes & Genet. Systems. 2013. V. 88. № 1. P. 69–76. https://doi.org/10.1266/ggs.88.69
3. Paulauskas A., Griciuvienė L., Juknelyte S. et al. Genetic diversity and population structure of raccoon dog (Nyctereutes procyonoides) in invaded areas // XMAT. 2014. P. 78. Antalya, Turkey. https://www.neobiota.eu/wp/wp-content/uploads/NEOBIOTA-2014-Abstract-Book.pdf
4. Nørgaard L.S., Mikkelsen D.M.G., Elmeros M. et al. Population genomics of the raccoon dog (Nyctereutes procyonoides) in Denmark: Insights into invasion history and population development // Biol. Invasions. 2017. V. 19. № 5. P. 1637–1652. https://doi.org/10.1007/s10530-017-1385-5
5. Oh S.Y., Kim S.A., Kim J.Y. et al. Detection of antibodies against the rabies virus in Korean raccoon dogs (Nyctereutes procyonoides koreensis) // J. Zoo and Wildlife Med. 2012. V. 43. № 1. P. 174–176. https://doi.org/10.1638/2011-0063.1