Derivation of Induced Pluripotent Stem Cells Line (RCPCMi009-A-1) with Knockout of the UBE2A Gene by CRISPR/Cas9 Genome Editing-Reference-Cited by-同舟云学术

Derivation of Induced Pluripotent Stem Cells Line (RCPCMi009-A-1) with Knockout of the UBE2A Gene by CRISPR/Cas9 Genome Editing

Published:2023-11-01 Issue:6 Volume:54 Page:405-414
ISSN:0475-1450
Container-title:Онтогенез
language:
Short-container-title:Ontogenez

Author:

Khomyakova E. A.¹,Fedorenko A. V.¹²,Surdina A. V.¹,Volovikov E. A.¹³,Belikova L. D.¹³,Zerkalenkova E. A.⁴,Lagarkova M. A.¹⁵,Bogomazova A. N.¹³⁵

Affiliation:

1. Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine

2. Skolkovo Institute of Science and Technology

3. Research Institute of Medical Genetics, Tomsk National Research Medical Center of the Russian Academy of Scieces

4. Rogachev Federal Scientific and Clinical Centre of Pediatric Hematology, Oncology and Immunology

5. Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Lopukhin Federal Research and Clinical Center of Physical and Chemical Medicine

Abstract

The deletions and mutations in the UBE2A gene cause X-linked mental retardation syndrome of Nascimento type first described in 2006 (Nascimento et al., 2006). To study the role of the UBE2A gene in neurodevelopment, we generated a human iPSC line with knockout of the UBE2A gene (RCPCMi009-A-1) using genome editing CRISPR/Cas9 technology. The knockout of the UBE2A gene was confirmed by Western blotting. The pluripotent state of the RCPCMi009-A-1 iPSCs line was confirmed by typical stem cell morphology, normal male karyotype maintenance, expression of pluripotency markers and the ability to differentiate into three germ layers.

Publisher

The Russian Academy of Sciences

Reference10 articles.

1. Bogomazova A.N., Lagarkova M.A., Tskhovrebova L.V. et al. Error-prone nonhomologous end joining repair operates in human pluripotent stem cells during late G2 // Aging (Albany NY). 2011. V. 3. № 6. P. 584.

2. Concordet J.P., Haeussler M. CRISPOR: intuitive guide selection for CRISPR/Cas9 genome editing experiments and screens // Nucleic Acids Research. 2018. V. 46. № W1. P. W242–W245.

3. Czepulkowski B., Bhatt B., Rooney D. Malignancy and acquired abnormalities. Vol. 2. Oxford University Press. New York. 1992. V. 2. P. 1–25.

4. Dover J., Schneider J., Tawiah-Boateng M.A. et al. Methylation of histone H3 by COMPASS requires ubiquitination of histone H2B by Rad6 // J. Biological Chemistry. 2002. V. 277. № 32. P. 28368–28371.

5. Hibbert R.G., Huang A., Boelens R. et al. E3 ligase Rad18 promotes monoubiquitination rather than ubiquitin chain formation by E2 enzyme Rad6 // Proceedings of the National Academy of Sciences. 2011. V. 108. № 14. P. 5590–5595.

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