Phylogenetic relationships of <i>Mesocestoides</i> vaillant, 1863 tetrathyridia from small mammals of Eastern Russia and Alaska based on <i>18S rRNA</i> gene

Author:

Pospekhova N. A.1,Pereverzeva V. V.1,Dokuchaev N. E.1,Primak A. A.1

Affiliation:

1. Institute of Biological Problems of the North, Far Eastern Branch of the Russian Academy of Sciences

Abstract

A fragment of 875 bp of the 18S rRNA gene was studied in 12 samples of Mesocestoides tetrathyridia from small mammals of 7 species collected in geographically distant localities. Five haplotypes were identified, differing from each other by 27 nucleotide substitutions in 24 sites. It has been found that the 18S rRNA haplotypes belong to two genetically distinct haplogroups. Molecular diversity indices were calculated for each of them. The conducted analysis allowed to suggest the following: 1) Mesocestoides sp. haplogroups A and B belong to two different species, but do not belong to any of the confirmed species of the genus; 2) the deletion of bp 729–747 and the insertion at site 761 of guanine can be regarded as a genetic marker for the species Mesocestoides litteratus.

Publisher

The Russian Academy of Sciences

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