Biochip with Cells of Brush Polymers Carrying Carboxyl Groups for DNA Analysis-Reference-Cited by-同舟云学术

Biochip with Cells of Brush Polymers Carrying Carboxyl Groups for DNA Analysis

Published:2023-11-01 Issue:6 Volume:49 Page:641-648
ISSN:1998-2860
Container-title:Биоорганическая химия
language:
Short-container-title:RUBI

Author:

Miftakhov R. A.¹,Nasedkina T. V.¹,Zasedatelev A. S.¹,Shershov V. E.¹,Kuznetsova V. E.¹,Levashova A. I.¹,Lapa S. A.¹,Vasiliskov V. A.¹,Ikonnikova A. Yu.¹,Chudinov A. V.¹

Affiliation:

1. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences

Abstract

A method for manufacturing biochips by photolithography with hydrogel cells made of brush copolymers based on acrylic acid and acrylamide, fixed at one end on the surface of a polymer substrate has been developed. Hydrogel cells with reactive carboxyl groups were used for covalent immobilization of oligonucleotide probes. The effectiveness of the method was demonstrated in the hybridization analysis of DNA by targets of different lengths of the sequence site 7 of the exon of the human ABO gene.

Publisher

The Russian Academy of Sciences

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