Synthesis of Fluorescent-Labeled Nucleotides for Labeling of Isothermal Amplification Products-Reference-Cited by-同舟云学术

Synthesis of Fluorescent-Labeled Nucleotides for Labeling of Isothermal Amplification Products

Published:2023-11-01 Issue:6 Volume:49 Page:649-656
ISSN:1998-2860
Container-title:Биоорганическая химия
language:
Short-container-title:RUBI

Author:

Shershov V. E.¹,Kuznetsova V. E.¹,Zasedatelev A. S.¹,Vasiliskov V. A.¹,Shekalova E. Yu.¹,Shtylev G. F.¹,Shishkin I. Yu.¹,Levashova A. I.¹,Lapa S. A.¹,Chudinov A. V.¹

Affiliation:

1. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences

Abstract

The synthesis of fluorescently labeled nucleotides containing a zwitterionic indodicarbocyanine dye attached via a trans-alkene spacer at the C5 position of the pyrimidine base was carried out, and their substrate efficiency was tested under conditions of recombinase polymerase amplification (RPA). As a result of RPA, the formation of full-sized target products of the ebpS gene fragment of the causative agent of bacterial pneumonia (Staphylococcus aureus) and a high density of fluorescent label embedding occurred.

Publisher

The Russian Academy of Sciences

Reference17 articles.

1. Donatin E., Drancourt M. // Med. Mal. Infect. 2012. V. 42. P. 453–459. https://doi.org/10.1016/j.medmal.2012.07.017

2. Erdmann V.A., Jurga S., Barciszewski J. // RNA DNA Diagnostics. 2015. P. 259–280. https://doi.org/10.1007/978-3-319-17305-4_13

3. Huang H.-J., Campana R., Akinfenwa O., Curin M., Sarzsinszky E., Karsonova A., Riabova K., Karaulov A., Niespodziana K., Elisyutina O., Fedenko E., Litovkina A., Smolnikov E., Khaitov M., Vrtala S., Schlederer T., Valenta R. // Front. Immunol. V. 11. P. 594978. https://doi.org/10.3389/fimmu.2020.594978

4. Erickson D., O’Dell D., Jiang L., Oncescu V., Gumus A., Lee S., Mancuso M., Mehta S. // Lab. Chip. 2014. V. 14. P. 3159–3164. https://doi.org/10.1039/C4LC00142G

5. Temiz Y., Lovchik R.D., Kaigala G.V., Delamarche E. // Microelectronic Engineering. 2015. V. 132. P. 156–175. https://doi.org/10.1016/j.mee.2014.10.013