Synthesis of steroid tracers with a oxyme ligation method and use thereof in fluorescent polarisation immunoassay-Reference-Cited by-同舟云学术

Synthesis of steroid tracers with a oxyme ligation method and use thereof in fluorescent polarisation immunoassay

Published:2024-08-19 Issue:1 Volume:50 Page:51-64
ISSN:1998-2860
Container-title:Биоорганическая химия
language:
Short-container-title:RUBI

Author:

Prokhorenko I. A.¹,Glushchenko D. A.¹,Gulyak E. L.¹,Mikhura I. V.¹,Korshun V. A.¹,Mukhametova L. I.²,Eremin S. А.²

Affiliation:

1. Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry RAS

2. Lomonosov Moscow State University

Abstract

A method for the synthesis of ketosteroid oxime conjugates with a fluorescein (6-FAM) derivative connected via a short bifunctional aminooxypropyl linker is proposed. The conjugates were used as tracers for fluorescence polarization immunoassay (FPIA) with monoclonal antibodies. Stereoisomers by the oxime group were separated by preparative RP TLC and HPLC and tested in the FPIA method. Binding parameters of of tracers with monoclonal antibodies (mAbs) to progesterone were studied. Tracers showed high efficiency in the analysis of steroid hormones, the detection limit of progesterone being lower than that for a previously described analog. The Z-isomer was found to be more sensitive in the FPIA analysis compared to the E-isomer.

Publisher

The Russian Academy of Sciences

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