Affiliation:
1. Shenzhen MSU-BIT University, International University Park Road 1
2. Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
3. Interdisciplinary Scientific and Educational School of Moscow University “Molecular Technologies of the Living Systems and Synthetic Biology”, Faculty of Biology, Lomonosov Moscow State University
Abstract
A recombinant analog of the human SLURP-1 protein (rSLURP-1) effectively inhibits the growth of carcinomas by interaction with the α7-type nicotinic acetylcholine receptor. Recently, rSLURP-1 inhibition of gliomas growth in vitro was shown by the authors, although, the mechanism of rSLURP-1 action was not studied. Here, we showed that rSLURP-1 selectively inhibits the growth of U251 MG glioma cells but not of normal astrocytes, and controls glioma cell migration. In addition, rSLURP-1 induces cell cycle arrest in the G2/M phase in U251 MG glioma cells, but does not result in apoptosis. Incubation of U251 MG cells with rSLURP-1 causes inhibition of phosphorylation of ERK, p38 MAPK, and AKT kinases, the activation of which contributes to the progression of gliomas. At the same time, rSLURP-1 does not affect the activity of JNK kinase. Thus, rSLURP-1 is an endogenous protein promising for the development of drugs based on it for the treatment of not only carcinomas, but also gliomas.
Publisher
The Russian Academy of Sciences