Inhibitory Effect of Oxibiol on the Process of Protein Modification by Water-Soluble Products of Photo-Oxidative Destruction of Bisretinoid A2E

Abstract

It has previously been shown that heteroaromatic antioxidant oxibiol (N-acetylcysteinate 6-hydroxy-2-aminobenzothiazole) inhibits the process of fructosylation of serum albumin. The aim of this study is to elucidate the inhibitory effect of oxibiol on modification of proteins by photodegradation products of bisretinoid A2E, the main fluorophore of lipofuscin granules in retinal pigment epithelial cells of the human eye. It was shown that unlike a water-soluble fraction fraction from non-irradiated A2E-liposomes, a water-soluble fraction formed after irradiation of A2E-liposomes with visible light significantly modified albumin in a day after incubation at 37°C. Oxibiol in millimolar concentrations effectively inhibited this process. The inhibitory effect of oxybiol could be attributed to its antioxidant activity and the ability to compete with reactive aldehydes formed during the photooxidative degradation of A2E-cardiolipin liposomes. The acute toxicity of oxibiol in mice after intraperitoneal injection was studied, the values of LD 10 and LD 50 were determined. The results obtained demonstrate that oxibiol can be used in pharmacology for preventing and curing diseases associated with the development of oxidative stress in various fields of medicine, primarily in ophthalmology.