Effect of the nitric oxide synthesis inhibitor L-NAME on the isolated rat heart after hypokinesia-Reference-Cited by-同舟云学术

Effect of the nitric oxide synthesis inhibitor L-NAME on the isolated rat heart after hypokinesia

Published:2023-08-15 Issue:4 Volume:68 Page:754-760
ISSN:0006-3029
Container-title:Биофизика
language:
Short-container-title:Biofizika

Author:

Sungatullina M. I¹,Zaripova R. I¹,Ziyatdinova N. I¹,Yafarova G. G¹²,Andrianov V. V¹²,Gainutdinov Kh. L¹²,Zefirov T. L¹

Affiliation:

1. Kazan Federal University

2. Zavoisky Physical-Technical Institute - Subdivision of Kazan Scientific Center, Russian Academy of Sciences

Abstract

The effects of a non-selective nitric oxide synthase inhibitor L-NAME on the functional parameters of the isolated rat heart after a 30-day period of hypokinesia were studied. Electron paramagnetic resonance spectroscopy was employed in the analysis of a role for L-NAME in the intensity of nitric oxide production in rat heart tissues. The intensity of nitric oxide synthesis was assessed by the intensity of the signal belonging to the (DETC)2-Fe2+-NO complex. It was found that L-NAME decreased nitric oxide production on average by 69%. The Langendorff isolated perfused heart was used to evaluate cardiac activity, and the following parameters were measured: pressure generated by the left ventricle, heart rate, and coronary flow. Addition of the nitric oxide synthesis inhibitor L-NAME induced an increase in inotropic function and normalization of heart rate.

Publisher

The Russian Academy of Sciences

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