Application of various growth regulators in clonal micropropagation of black currant (Ribes Nigrum L.)

Abstract

Currently, the optimization and development of new techniques for microclonal reproduction of black currant (Ribes nigrum L.) are one of the traditional objects of research of domestic and foreign scientists. It is noted that the success of reproduction depends on a number of factors: the timing of introduction into culture in vitro, the type of explants, the sterilizing agent, the composition of the nutrient medium. At the same time, the survival and growth of explants at each stage of reproduction depends not only on the salt composition of the nutrient medium, but also on growth regulators, mainly cytokines and auxins. This article discusses the theoretical aspects of the use of various growth regulators at different stages of clonal micropropagation of black currant, provides methods of microclonal reproduction developed by scientists of leading research organizations.