Aptasensors based on track-etched membranes coated with nanostructured silver layer for influenza A and B virus detection

Author:

Kukushkin V. I.1,Kristavchuk О. V.2,Zhdanov G. А.3,Keshek А. К.3,Gambaryan А. S.4,Andreev Ye. V.2,Nechaev A. N.2,Zavyalova Е. G.3

Affiliation:

1. Institute of Solid State Physics of the Russian Academy of Sciences

2. Joint Institute for Nuclear Research

3. Lomonosov Moscow State University

4. Chumakov Federal Scientific Centre for Research and Development of Immune and Biological Products of the Russian Academy of Sciences

Abstract

A biosensor based on a polyethylene terephthalate track membrane coated with silver nanoparticles is proposed for the detection of influenza A and B viruses using aptamers for specific sorption of the viruses on the membrane surface, as well as for the introduction of a Raman-active or fluorescent label in the complex. The analytical signal was recorded using a Raman spectrometer, observing the effects of surface-enhancing of the intensity of optical responses from the labels.

Publisher

The Russian Academy of Sciences

Reference34 articles.

1. Кукушкин В.И., Гришина Я.В., Соловьев В.В. и др. // Письма ЖЭТФ. 2017. Т. 105. С. 637

2. Kukushkin V.I., Grishina Y.V., Solov'ev V.V. et al. // JETP Lett. 2017. V. 105. P. 677.

3. Кукушкин В.И., Гришина Я.В., Егоров С.В. и др. // Письма ЖЭТФ. 2016. Т. 103. С. 572

4. Kukushkin V.I., Grishina Y.V., Egorov S.V. et al. // JETP Lett. 2016. V. 103. P. 508.

5. Белик А.Я., Кукушкин В.И., Рыбин А.Я. и др. // Докл. РАН. 2018. Т. 481. С. 270

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