Affiliation:
1. Lomonosov Moscow State University
2. Koltzov Institute of Developmental Biology, Russian Academy of Sciences
Abstract
Serotonin is a regulator of early embryonic development and has a fully functional system in preimplantation mammalian embryos. In this work, we described the spatial distribution of serotonin, the vesicular monoamine transporter VMAT2, and the serotonin receptors 5-HT1D and 5-HT2A at different stages of early mouse embryonic development. Serotonin, the VMAT2 transporter, and the 5-HT1D receptor are visualized in the cortical compartment of cells, whereas the 5-HT2A receptor has a more uniform distribution throughout the cytoplasm. Comparison of the sizes of the immunoreactive particles showed that there are no statistically significant differences between serotonin and the VMAT2 transporter, which may indicate the presence of specific vesicles containing serotonin. In addition, the two serotonin receptors, 5-HT1D and 5-HT2A, showed a markedly different pattern of immunoreactivity, which may indicate that they simultaneously serve different functions in early embryogenesis.
Publisher
The Russian Academy of Sciences
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