Assessment of the Toxic Effect of 2-(Chlorodinitromethyl)-4-Methoxy-6-(4-Methylpiperazine-1-yl)-1,3,5-Triazine by Respiratory Activity of Lymphocytes-Reference-Cited by-同舟云学术

Assessment of the Toxic Effect of 2-(Chlorodinitromethyl)-4-Methoxy-6-(4-Methylpiperazine-1-yl)-1,3,5-Triazine by Respiratory Activity of Lymphocytes

Published:2023-09-01 Issue:5 Volume:40 Page:342-350
ISSN:0233-4755
Container-title:Биологические мембраны Журнал мембранной и клеточной биологии
language:
Short-container-title:Biologičeskie membrany

Author:

Iliasov P. V.¹,Limareva L. V.¹,Sizova A. I.¹,Zalomlenkov V. A.²,Kuricyna A. P.¹

Affiliation:

1. Samara State Medical University, Ministry of Health of the Russian Federation

2. Samara State Technical University

Abstract

A method for evaluation of metabolic characteristics of intact cells based on electrochemical registration of their respiratory activity was used to monitor a reaction of lymphocytes to a potential pharmacological agent, 2-(chlorodinitromethyl)-4-methoxy-6-(4-methylpiperazin-1-yl)-1,3,5-triazine. The method ensured an estimation of cytotoxicity of the test compound and made it possible to determine its minimum toxic concentrations for human lymphocytes. It was shown that the obtained results agree with the data of a reference method – MTT-based cell viability assay.

Publisher

The Russian Academy of Sciences

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