Microbial 1,2-dehydrogenation of 6 Alpha-Methylhydrocortisone 11-trifluoroacetate-Reference-Cited by-同舟云学术

Microbial 1,2-dehydrogenation of 6 Alpha-Methylhydrocortisone 11-trifluoroacetate

Published:2023-11-01 Issue:6 Volume:59 Page:622-630
ISSN:0555-1099
Container-title:Прикладная биохимия и микробиология
language:
Short-container-title:Prikladnaâ biohimiâ i mikrobiologiâ

Author:

Savinova T. S.¹,Arinbasarova A. Yu.²,Kazantsev A. V.³,Savinova O. S.⁴,Lukashev N. V.³

Affiliation:

1. Erisman FSCH of Rospotrebnadzor

2. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Federal Research Center “Pushchino Scientific Center for Biological Research of the Russian Academy of Science”

3. Lomonosov Moscow State University, Faculty of Chemistry

4. Bach Institute of Biochemistry, Biotechnology Research Center of Russian Academy of Sciences

Abstract

Transformation of 11-trifluoroacetate 6α-methylhydrocortisone (11-TFA MHC) by cells of actinobacteria Arthrobacter (Nocardioides) was carried out in the presence of α-cyclodextrin (α-CD). The composition and dynamics of the transformation products accumulation in the culture medium at various pH values and the ratio of α-CD/substrate were studied. It was shown that the addition of α-CD to the transformation medium at pH less than 7 promotes an increase in the rate of 1,2-dehydrogenation with the formation of 6 α-methylprednisolone 11-trifluoroacetate (11-TFA MPL). At pH over 7, the primary process is the hydrolysis of the 11β-trifluoroacetyloxy group. In this case, the participation of α-CD in these processes as an acceptor of the trifluoroacetyl ion is not excluded.

Publisher

The Russian Academy of Sciences

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