Kinetic Peculiarities of Nucleic Acid Elongation as a Multistage Cosequent Enzymatic Reaction-Reference-Cited by-同舟云学术

Kinetic Peculiarities of Nucleic Acid Elongation as a Multistage Cosequent Enzymatic Reaction

Published:2023-03-01 Issue:2 Volume:64 Page:181-188
ISSN:0453-8811
Container-title:Кинетика и катализ
language:
Short-container-title:Kinetika i kataliz

Author:

Lukovenkov A. V.¹,Bykov V. I.¹²,Varfolomeev S. D.¹²³

Affiliation:

1. Emanuel Institute of Biochemical Physics, Russian Academy of Sciences

2. Lomonosov Moscow State University, Institute of Physicochemical Foundations of the Functioning of Neural Network and Artificial Intellegence

3. Lomonosov Moscow State University, Faculty of Chemistry, Department of Chemical Ensymology

Abstract

The article investigates kinetics of nucleic acid elongation as a multistage consequent reaction curling up to cycle. There is represented a matematically precise proof of empiric formulae used to estimate elongation time depending on nucleotide chain length. There are given some estimations of characteristic elongation time for typical chain lengths, for example for new coronavirus (SARS-nCoV-2). There is also investigated stability of elongation kinetics and at typical chain lenghts is shown an existance of instable oscillating solution component in addition to main exponential component.

Publisher

The Russian Academy of Sciences

Reference19 articles.

1. Mullis K.B., Smith M. The Polymerase Chain Reaction / Stockholm: Nobel media AB. Nobel Lecture. December 8, 1993. http://www.nobelprize.org/nobel_prizes/chemistry/laureates/1993/mullis-lecture.html

2. Saiki R., Scharf S., Faloona F., Mullis K., Horn G., Erlich H., Arnheim N. Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia // Science. 1985. V. 230. P. 1350.

3. Saiki R., Gelfand D., Stoffel S., Scharf S., Higuchi R., Horn G., Mullis K., Erlich H. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase // Science. 1988. V. 239. № 4839. P. 487.

4. Severe acute respiratory syndrome coronavirus 2 isolate Wuhan-Hu-1, complete genome. https://www.ncbi.nlm.nih.gov/nuccore/MN908947.3.

5. Higuchi R., Fockler C., Dollinger G., Watson R. Kinetic PCR Analysis. Real-time Monitoring of DNA Amplification Reactions // Biotechnology. 1993. № 11. P. 1025.