Development and Application of Two Multiplexes of Nuclear Microsatellite Loci for the Analysis of Genetic Variability of Scots Pine Populations in Different Parts of the Range-Reference-Cited by-同舟云学术

Development and Application of Two Multiplexes of Nuclear Microsatellite Loci for the Analysis of Genetic Variability of Scots Pine Populations in Different Parts of the Range

Published:2023-07-01 Issue:4 Volume: Page:427-433
ISSN:0024-1148
Container-title:Лесоведение
language:
Short-container-title:Lesovedenie

Author:

Semerikov N. V.¹

Affiliation:

1. Botanical Garden, Ural Branch of the RAS

Abstract

Multiplexing of microsatellite loci (SSR) can significantly reduce the cost and duration of the analysis. Based on the published microsatellites of Scots pine (Pinus sylvestris L.), we developed and tested two multiplexes of 14 loci on seven populations from different parts of the range. Genetic variability was revealed in all populations. The average number of alleles was 5.78, the average expected heterozygosity was 0.641. Significant interpopulation differentiation at the level of 1.8% was revealed. In all loci, the mean frequencies of null alleles did not exceed 7.1%. The results of the genetic analysis of populations confirm the suitability of the resulting multiplexes for population genetic studies of Scots pine.

Publisher

The Russian Academy of Sciences

Reference22 articles.

1. Калько Г.В., Котова Т.М. Микросателлитные маркеры для оценки генетического разнообразия сосны обыкновенной // Труды Санкт-Петербургского научно-исследовательского института лесного хозяйства. 2018. № 3–4. С. 17–30.

2. Правдин Л.Ф. Сосна обыкновенная. М.: Наука, 1964. 190 с.

3. Санников С.Н., Санникова Н.С., Петрова И.В. Очерки по теории лесной популяционной биологии. Екатеринбург: РИО УрО РАН, 2012. 271 с.

4. Шуваев Д., Ибе А., Щерба Ю., Сухих Т., Шилкина Е., Усова Е., Лисотова Е., Репях М., Ступакова О. Разработка панели ядерных микросателлитных локусов для оценки легальности происхождения древесины сосны обыкновенной в Красноярском крае // Хвойные бореальной зоны. 2020. Т. 38. № 5–6. С. 297–304.

5. Auckland L., Bui T., Zhou Y., Shepherd M., Williams C. Conifer microsatellite handbook. Raleigh: Corporate Press, 2002. 61 p.